What is Virus Plaque Assay

In this Video, Ms. Poonam Rajawat, Assistant Professor, Biyani Group of Colleges, describes about virus plaque assay. It is an assay to enumerate phage particle on solid agar media. A clear area formed on agar media known as Plaque. It is a double layered technique. Phage dilution was prepared in trypton broth and two drops of E. coli. were added when bacteriophage affect the bacteria, it causes lytic cycle and form plaque.